Blogs/News/PSAs

The Gyrlfriend Code Sorority Edition is going on tour! DMV, you will be the first stop. Please secure your tickets TODAY! Come out and hear stories of TRAILBLAZERS from all Divine Nine Sororities discuss their Gyrlfriend relationships from a sorority perspective. If you have family/friends in the DMV area, please help us spread the word. The event is FREE but registration is required while space lasts. Thank you for your support and we hope to see you there!!! https://www.eventbrite.com/e/the-gyrlfriend-code-sorority-edition-official-book-signing-dmv-tickets-63785208275 #bestsellingauthor #dressiespeaks #publicfigure #author #blogger #motivationalspeaker #Educationfirst #publicspeaking #coach #entrepreneur #leader #businesswoman #CEO #cohost #professor #president #founder #director #strategist #chairwoman #yourdreamisyourdream #impacteducation #purpose #plan #destiny #theheartofschooltransformation #TheGyrlfriendCodeSororityEdition #AlphaKappaAlpha

Prayer is both valuable and necessary but we also need to do something practical that the Lord can bless and use for the sake of others to grow in His kingdom. 
 

When Jehoshaphat was surrounded by a great army from Moab, Ammon and Mt Seir he enquired of the Lord and prayed publicly to the Lord, when the prophet gave him God’s word, This is what the Lord says to you: ‘Do not be afraid or discouraged because of this vast army. For the battle is not yours, but God’s. 16Tomorrow march down against them.  …  Do not be afraid; do not be discouraged. Go out to face them tomorrow, and the Lord will be with you. 
After consulting the people, Jehoshaphat appointed men to sing to the Lord and to praise him for the splendor of his ^c holiness as they went out at the head of the army, saying:

“Give thanks to the Lord,

for his love endures forever.”

22As they began to sing and praise, the Lord set ambushes against the men of Ammon and Moab and Mount Seir who were invading Judah, and they were defeated. Read the whole story in 2 Chronicles 20

I want you to note:-

• They prayed.
• They had a direct word from God.
• They consulted the people. They had a strategy.
• They put the singers and musicians in front of the army.
• The enemy destroyed themselves.
• Jeshoshaphat didn’t even have to fight, but they did have to work, it took 3 days to carry off the plunder. 

Think about Joshua walking around the walls of Jericho, they heard from God, they had a strategy, they had to do something, but then the walls came down without any battering ram or artillery, still the Israelites had to go in and fight and take possession of the city.

There are too many Christians today playing around with prayer and not hearing from God, getting a strategy, doing the work or getting into the fight. 
If you pray first, seek His will and guidance then when He gives you a strategy, go out and fight. God will give you the victory, stop waiting for some giant blessing of money, land, buildings, or other resources to drop on you without exercising a God given strategy.

Maybe it could be start a business, visit a certain place, make a gift to someone, do some physical work, God honours faith that has legs, make sure you do what He instructs because the danger is thinking that your works may get your blessing, no, it is doing His work, as instructed by Him, that’s gets His blessing. 

Can your knowledge, experience, skill, anointing be useful to help other leaders - they are waiting to hear from you and we welcome you to contribute to maturity and unity across the African church - just click the link, sign up and start sharing some powerful insights.here.   http://bftfafricanetwork.ning.com/

 Chicago's very own “ Brandon James" is ready for success, and he wants you to know it. The r&b/soul singer is creating a strong buzz with the upcoming release of his single "So Emotional." "So Emotional,"  is scheduled for release on April 26th, 2019 and is the first single off the upcoming E.P scheduled for release this fall. 

 Chicago native Brandon James was born "Brandon James Foster". He states that he "started doing music as an outlet for depression early in life. I wasn't very popular in school and I was considered a latchkey kid due to my mother's work schedule and my father's battles with alcoholism." On a teacher's suggestion to find extracurricular activities, Brandon joined the choir. He finished the eighth grade with a determination to elevate his understanding of music and succeed with his own music. Constantly challenging himself to become better at his craft, he has taken voice lessons from Bobby Wilsyn, Ars Musica, and Andrew Schultze. He has played with Earth, Wind & Fire and performed at some of Chicago’s most notable venues including the UIC Pavilion and the House of Blues. Brandon has also earned a degree in music performance and business from the Columbia College of Chicago. Forever seeking to share his story and testimony, Brandon seeks to inspire, touch hearts and provoke thought with his music..
Links:
https://show.co/hwfa7YD
https://show.co/nDQNCKx
https://open.spotify.com/album/4jM905OI0YxTH0g2l1mb34
https://itunes.apple.com/us/album/so-emotional-single/1458270056
https://soundcloud.com/branddo20/so-emotional-master
https://www.youtube.com/watch?v=u-d2LgEOrIE&feature=youtu.be
https://brandonjames.storyamp.com/
https://show.co/beJy4Km
http://www.branddo20music.com/p/so-emotional.html
https://open.spotify.com/album/4jM905OI0YxTH0g2l1mb34
https://itunes.apple.com/us/album/so-emotional-single/1458270056
https://soundcloud.com/branddo20/so-emotional-master
https://www.youtube.com/watch?v=u-d2LgEOrIE&feature=youtu.be
https://brandonjames.storyamp.com/

Joining a Sorority or already in a Sorority? Checkout the CODES and Get your Autographed copy of this Hot New Bestseller TODAY! Email at dressie@dressiespeaks.com or emmckoy@gmail.com (indicate number of autographed copies, include shipping address, name you want me to address the message to for only $19.99 plus $4.99 for shipping & handling) www.dressiespeaks.com (send information via contact) #bestsellingauthor #dressiespeaks #publicfigure #author #blogger #motivational-speaker #Educationfirst #publicspeaking #coach #entrepreneur #leader #businesswoman #CEO #cohost #professor #president #founder #director #strategist #chairwoman #yourdreamisyourdream #impacteducation #purpose #plan #destiny #theheartofschooltransformation #TheGyrlfriendCodeSororityEdition #AlphaKappaAlpha

Precise cell analysis for:

PBMCs, Car-T Cells, Whole Blood Cells

Stem Cells

Splenocytes

Monocytes

And Other Primary Cells

Product Overview

The Countstar S2 System combines the two fluorescence channels plus a bright field digital microscope, an image cytometer, and a cell counter in a single bench-top instrument. This application-driven, compact, automated cell imaging system provides an all-in-one solution for cell counting, cell viability and Cell transfection efficiency through the use of preconfigured biological applications (BioApps). They are specifically optimized for analysis of primary cells from peripheral blood, including PBMC, CAR-T, NK cell and MSC, which commonly used in cell therapy. NK cell activation assay.

Excitation light: 480nm, 525nm

Emission filter: 535nm, 600LP

Key Benefits

Automatic detecting 5 samples for one time

GMP and 21 CFR Part 11 ready

Multi channel, multi functions

Simple, effortless operation

Maintenance and service free

All in one design- Touch screen user interface

User friendly and flexible software

Product feature

Smart

The BioApps of the Countstar Rigel S2 System simplify routine cell lab tasks while providing high quality scientific data.

Countstar S2 comes with three BioApps to simplify and automate the routine cell count, viability and cytotoxicity tasks.

Trypan blue BioAppTrypan blue BioApp: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.

AO/PI viability protocolAO/PI Viability BioApp: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.

GFP TransfectionGFP transfection: The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP.

Three step process

The Countstar S2 System is designed to take you from sample to result in one go, simplifying your work and allowing for increased productivity and efficiency.

Reproducible and Accurate

Countstar S2 system can automatic detect 5 samples at one time, it will be more efficiency and affordable, with integrated patent “fixed focus”, the results would be more stable and reliable.

Touch Screen with Flexible Experiment Assay Management System

“App-like” interface. Allow to add multiple Experiment Assays for different operators

GMP ready

The Countstar S2 system is designed to meet the modern pharmaceutical requirements. The software complies with 21 CFR part 11. Key features include control of user access and data export, and secure audit trail. At the same time, we also supply IQ/OQ service and PQ support to help customer build a reliable system.

Specifications

Applications

Monitoring the Quality of CAR-T Production during the whole Process

The immuno therapy, such as CAR-T involves the collection of peripheral blood mononuclear cells (PBMC) from the patient, then enrichment of the desired T cell subset followed by genetic modification to create engineered CAR-T cells. The CAR-T cells are expanded in large-scale and then infused back into the patient. With the Countstar S2 system it is possible to continuously monitor the viability and concentration every step of the entire process of manufacturing CAR-T cells under GMP.

Dual-Fluorescence Viability

Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. AO can penetrate to both dead and live cells and stains the nucleated cells to generate green fluorescence. PI can stain the dead nucleated cells with compromised membranes and generate red fluorescence. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result. In conclusion, the Countstar S2 system can be used for every step of the cell manufacturing process.

GFP Transfection Efficiency

In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. Currently, scientists are commonly using the fluorescent microscopes or flow cytometers to analyze the transfection efficiency of mammalian cells. But Flow cytometer requires a high-qualified and experienced operator. While Countstar FL enables users to get the result of a transfection efficiency assay quickly and accurately.

Trypan blue viability and concentration

Trypan blue viability and concentration assay also can be available on Countstar S2. Shown here, are the enlarged representative images of cell line. Since all of the cells were stained with trypan blue, we show the stained trypan blue image as well as the Countstar S2 counted image.

Pensacola, Florida's Raven Salve' is the rising singer/songwriter that is sweeping the country with her angelic sound. From the Gulf Coast to all across the county, Raven Salve' has impacted the lives of music lovers of all ages with her incredible sound.


Raven Salve's musical talent spans over twenty years. With each CD release, she continues to show her ability to keep producing one hit song after another. Her latest project is entitled "METAMORPHOSIS THE TRANSFORMATION" and is the most epic project in her career so far. The album is a must have and definitely offers a variety of songs to everyone.


Raven will be hosting a album release party and masquerade ball in Atlanta, Ga on July, 13th. A party bus has been obtained for the event and will take fans and guest from Pensacola, FL to the release party in Atlanta. The bus will leave the Town & Country Plaza in Pensacola at 8 am on July, 13th. The destination will be Frankie Beverly's Backstage Atlanta. The after party will be at Raymond's House. The bus will return all fans and guest to The Town & Country Plaza in Pensacola at the end of the event/night. Food, liquor, your mask for the ball and round trip is all inclusive. 


Get your tickets now while they are still available:


https://ravensalve.com/


Call or text 850-610-2097 or 850-288-3755

The Gyrlfriend Sorority Edition!!! is almost here!!! It’s going to be EPIC!! Stay Tuned for more details!!!! Official Release Date: Thursday June 6, 2019!!!!

A Must See with the Hosts of Education First!

Tune in to The Renee’ Allen and Friends Show at www.listenvisionlive.com Wednesday, June 5, 2019 at 7 PM EST!

Tune in TODAY! Mark your calendars to hear all about The Grylfriend Code Sorority Edition Support, Collaboration, and Enrichment Codes with Co-authors Tiffani Willis, Tywauna Wilson, and Dr. Essie McKoy, today, June 4, 2019 at 7:30! PM EST! Email at dressie@dressiespeaks.com or emmckoy@gmail.com or go to www.dressiespeaks.com #dressiespeaks #publicfigure #author #blogger #motivationalspeaker #Educationfirst #publicspeaking #coach #entrepreneur #leader #businesswoman #CEO #cohost #professor #president #founder #director #strategist #chairwoman #yourdreamisyourdream #impacteducation #purpose #plan #destiny

“The Heart of School Transformation," is about the journey into the experience of Dr. Essie McKoy's path in the field of education. She gives effective strategies in how she has transformed schools to become Signature Schools. Dr. McKoy shares about her humble upbringing and how this led to her life's calling of serving students who may not have tangible hope and spoken dreams. In addition, this masterful book is designed to provide school leaders, educators, and district office personnel with tools and techniques to better equip them in preparing to turnaround challenging schools. Even those in higher education can use these research-based strategies to incorporate into their education programs to better prepare those entering the field of education. It is a book that will inspire and motivate not only educators and school leaders, but students, parents, local, state, and national leaders, and anyone willing to assist in the process of school transformation!

WIN Howard Sands' new book Crusade-Seminar Manual.

Africa Network BFTF invitation competition.

Policies and procedures for planning and running large scale events, crusades, seminars, events, expo's etc.

With almost 40 years of experience in being part of (speaking, planning, hosting) big events for outreach and leadership training in Australia and developing world countries, Howard Sands brings together the collective wisdom of many organisations to show you the practical steps to take, the timelines to work to, the important considerations for team members and gathering the best fruit from your event to make a Kingdom based, life and community changing event. Join Africa Network BFTF here to enter http://bftfafricanetwork.ning.com

NEXT BIG THING IN GOD'S MIND!

A global gathering of Kingdom eagles from more than 20 nations scheduled for the 20th-23rd of November in Dar es Salaam, Tanzania to seek the mind and heart of God on "The Building of the 21st-Century Church."

Workshop Topics Include:

I) Contending for the faith

ii) Repositioning the fivefold ministry

iii) Last Apostolic Invasion

iv) Prophetic Insight On the 21st Century Church

V) Winning the economic war (God’s financial plan for the 21st-century church)

VI) World mission strategies for the 21st century

VII) The Ministry of the Saints

VIII) The role of the church in national transformation, community development and in resolving global challenges

IX) The Ministry of the Holy Spirit today

X) The Internet revolution And the 21st Century Church

Activity is not a strategy! It is possible to be active without being productive. Brethren, we don't have time on our side, we can't afford to be operating without an effective strategy for real victory. In the absence of strategy, you can't avoid tragedy.

Come and discover Spirit-led strategy and insight for the building of the 21st-century church and the conquest of your city and nation for Christ.

You Don't Want To Miss! Click on the link below to register!

Online Registration: https://form.myjotform.com/62344954942564

Global Faith Coalitionwww.globalfaithcoalition.ning.com