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Polyacrylamide gel electrophoresis refers to the polyacrylamide gel that formed with the effect of acrylamide (abbreviated as Acr) and crosslinker N,N'-methylenebisacrylamide (abbreviated as Bis) under the action of catalyst ammonium persulfate (APS), N, N, N', N' tetramethylethylenediamine (TEMED) by polymeric cross-linking and used as a support for electrophoresis. 

Polyacrylamide gel electrophoresis can separate proteins into several zones according to the difference in charge of different protein molecules and the different mobility generated by different molecular sizes. If the sample contains only the same protein, only one band should be separated after the protein sample is electrophoresed. SDS is an anionic surfactant that breaks the hydrogen and hydrophobic bonds of a protein and combines it with a protein molecule to form a complex.

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Nitrification inhibitor refers to a class of chemical substances that inhibit the biotransformation of ammonium nitrogen into nitrate nitrogen (NCT). Nitrification inhibitors reduce the formation and accumulation of nitrate in soil so that it can reduce the loss of nitrogenous fertilizer in the form of nitrate nitrogen and the impacting on ecological environment. Some results show that, although nitrification inhibitor can reduce nitrogen leaching loss and greenhouse gas (nitrogen oxide) emission, it has a positive effect on improving fertilizer efficiency under certain conditions. However, nitrification inhibitors have not been widely used due to factors such as craftwork, cost and their own effects on the environment. It is necessary to search for some nitrification inhibitors which are effective in inhibiting nitrification and do not pollute the environment. 

Brief introduction of 3,4-Dimethylpyrazole Phosphate

• They can selectively inhibit the activity of nitrobacteria in soil. 
• Ammonium nitrogen can be absorbed by soil colloids and not easily lost. However, under the condition of soil permeability, ammonium nitrogen can be transformed into nitrate nitrogen under the action of microorganism, which is called nitrification.
• The speed of reaction depends on soil moisture and temperature. The speed of nitrification is very slow when it is below 10 °c. When the temperature is above 20 °C, the reaction rate is very fast. Except for some crops, such as rice, which can absorb ammonium nitrogen directly under irrigation, most crops absorb nitrate nitrogen. But nitrate is easy to be lost in soil. Rational use of nitrification inhibitors to control the rate of nitrification reaction can reduce the loss of nitrogen and increase nitrogen use efficiency. Nitrification inhibitors are usually mixed with nitrogen fertilizer and then applied.

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